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KMID : 0613820130230121495
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Journal of Life Science
2013 Volume.23 No. 12 p.1495 ~ p.1500
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Effects of Hizikia fusiforme Fractions on Melanin Synthesis in B16F10 Melanoma Cells
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Choi Eun-Ok
Kim Hyang-Suk
Han Min-Ho
Choi Yung-Hyun
Park Cheol
Kim Byung-Woo
Hwang Hye-Jin
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Abstract
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The objective of this study was to evaluate the anti-melanogenic effects of Hizikia fusiforme (HF) fractions in ${\alpha}$ 수식 이미지-melanocyte stimulating hormone-induced B16F10 mouse melanoma cells. Ethanol extractions of Hizikia fusiforme (EEHF) were subjected to fraction by using dichloromethane (CFHF), ethyl acetate (EAFHF), butanol (BFHF), and water (WFHF). EEHF, CFHF, and EAFHF inhibited tyrosinase activity and melanin synthesis in B16F10 mouse melanoma cells in a dose-dependent manner. The melanin contents were inhibited by 40.5% and 33.2% in response to treatment with 50 μg/ml of EEHF and CFHF, respectively. In addition, tyrosinase activities showed a 53.3% and 54.1% reduction in treatment with 50 μg/ml of EEHF and CFHF. Western blotting analysis showed that EEHF, CFHF, and EAFHF inhibited tyrosinase, TRP-1, TRP-2, and MITF expression in a dose-dependent manner. In conclusion, these findings indicate that ethanol and dichloromethane fractions of Hizikia fusiforme, which inhibit melanin synthesis and tyrosinase activity, are effective skin-whitening agents.
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KEYWORD
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Hizikia fusiforme, melanogenesis, tyrosinase
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